The Effects of Diets Including Different Amount of <em>Tribulus terrestris</em> Supplementation on Spermatological Parameters and Fertilization Ability of Male Rainbow Trout (<em>Oncorhynchus mykiss</em>)

Denizhan Kılınç, Bülent Yorulmaz & Fatih Öğretmen

doi:https://doi.org/10.29329/actanatsci.2023.353.07

Original article | Acta Natura et Scientia 2023, Vol. 4(1) 68-77

The Effects of Diets Including Different Amount of Tribulus terrestris Supplementation on Spermatological Parameters and Fertilization Ability of Male Rainbow Trout (Oncorhynchus mykiss)

Denizhan Kılınç, Bülent Yorulmaz & Fatih Öğretmen

pp. 68 - 77 | DOI: https://doi.org/10.29329/actanatsci.2023.353.07 | Manu. Number: MANU-2305-02-0006.R1

Published online: June 19, 2023 | Number of Views: 65 | Number of Download: 289

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Abstract

The aim of this study was to examine how varying doses of Tribulus terrestris supplementation affected the fertilization ability and spermatological characteristics of male rainbow trout (Oncorhynchus mykiss). Male fish (mean weight of 497±5.29 g and a mean length of 33.5±3.4 cm) were fed with four experimental diets containing different amounts of T. terrestris (0, 100, 250, 500 mg/kg feed) for 90 days in duplicates. The motility time analysis of all groups indicates that the motility times of male rainbow trout fed with T. terrestris increased. Besides, the fertilization rate of the control group is lower than the groups that fed with T. terrestris supplementation. In comparison with the control group without any additives, especially the fertilization rate of the trout fed with 250 mg/kg was 95% (p<0.05). Furthermore, this study also examined how T. terrestris supplementation affects the blood plasma of male rainbow trout and it was found that the male rainbow trout fed with T. terrestris supplementation had the highest LH hormone value at 250 mg/kg.

Keywords: Tribulus terrestris, Rainbow trout (Oncorhynchus mykiss), Seminal plasma, Sperm quality, Sperm motility

How to Cite this Article?

APA 6th edition
Kilinc, D., Yorulmaz, B. & Ogretmen, F. (2023). The Effects of Diets Including Different Amount of Tribulus terrestris Supplementation on Spermatological Parameters and Fertilization Ability of Male Rainbow Trout (Oncorhynchus mykiss) . Acta Natura et Scientia, 4(1), 68-77. doi: 10.29329/actanatsci.2023.353.07

Harvard
Kilinc, D., Yorulmaz, B. and Ogretmen, F. (2023). The Effects of Diets Including Different Amount of Tribulus terrestris Supplementation on Spermatological Parameters and Fertilization Ability of Male Rainbow Trout (Oncorhynchus mykiss) . Acta Natura et Scientia, 4(1), pp. 68-77.

Chicago 16th edition
Kilinc, Denizhan, Bulent Yorulmaz and Fatih Ogretmen (2023). "The Effects of Diets Including Different Amount of Tribulus terrestris Supplementation on Spermatological Parameters and Fertilization Ability of Male Rainbow Trout (Oncorhynchus mykiss) ". Acta Natura et Scientia 4 (1):68-77. doi:10.29329/actanatsci.2023.353.07.

References

Bhatia, D. K., Sharma, A. K., Pathania P. C., & Khanduri, N. C. (2010). Antifertility effects of crude different of Adiantum lunulatum Burm. on reproductive organs of male albino rats. Biological Forum- An International Journal, 2(2), 88-93. [Google Scholar]
Bhuvaneswari, R., & Balasundaram, C. (2006). Traditional Indian herbal extracts used in vitro against growth of the pathogenic bacteria – Aeromonas hydrophila. The Israeli Journal of Aquaculture-Bamidgeh, 58(2), 89-96. [Google Scholar]
Borges, A., Siqueira, S. R., Jurinitz, D. F., Zanini, R., Amaral, F., Grillo, M. L., Oberst, E. R., & Wassermann, G. F. (2005). Biochemical composition of seminal plasma and annual variations in semen characteristics of jundiá Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiology and Biochemistry, 31, 45-53. https://doi.org/10.1007/s10695-005-4742-8 [Google Scholar] [Crossref]
Bucci, L. R. (2000). Selected herbals and human exercise performance. American Journal of Clinical Nutrition, 72(2 Suppl), 624S-636S. https://doi.org/10.1093/ajcn/72.2.624s [Google Scholar] [Crossref]
Cek, S., Turan, F. & Atik, E. (2007). The effects of Gokshura, Tribulus terrestris on sex reversal of guppy, Poecilia reticulata. Pakistan Journal of Biological Sciences, 10(5), 718-725. https://doi.org/10.3923/pjbs.2007.718.725 [Google Scholar] [Crossref]
Chakraborty, S. B., Molnár, T., Ardó, L., Jeney, G., & Hancz, C. (2015). Oral administration of Basella alba leaf methanol extract and genistein enhances the growth and non-specific immune responses of Oreochromis niloticus. Turkish Journal of Fisheries and Aquatic Sciences, 15(1), 167–173. https://doi.org/10.4194/1303-2712-v15_1_18 [Google Scholar] [Crossref]
Citarasu, T. (2010). Herbal biomedicines: A new opportunity for aquaculture industry. Aquaculture International, 18(3), 403-414. https://doi.org/10.1007/s10499-009-9253-7 [Google Scholar] [Crossref]
Dimitrova, D. Z., Obreshkova, D., & Nedialkov, P. (2012). Antioxidant activity of Tribulus terrestris-a natural product in infertility therapy. International Journal of Pharmacy and Pharmaceutical Sciences, 4(4), 508-511. [Google Scholar]
Elsabagh, M., Mohamed, R., Moustafa, E. M., Hamza, A., Farrag, F., Decamp, O., & Eltholth, M. (2018). Assessing the impact of Bacillus strains mixture probiotic on water quality, growth performance, blood profile and intestinal morphology of Nile tilapia. Oreochromis niloticus, Aquaculture nutrition, 24(6), 1613-1622. https://doi.org/10.1111/anu.12797 [Google Scholar] [Crossref]
Ganzera, M., Bedir, E., & Khan, I. A. (2001). Determination of steroidal saponins in Tribulus terrestris by reversed-phase high-performance liquid chromatography and evaporative light scattering detection. Journal of Pharmaceutical Sciences, 90(11), 1752-1758. https://doi.org/10.1002/jps.1124 [Google Scholar] [Crossref]
Gauthaman, K., Adaikan, P. G., & Prasad, R. N. V. (2002). Aphrodisiac properties of Tribulus terrestris extract (Protodioscin) in normal and castrated rats. Life Sciences, 71(12), 1385-1396. https://doi.org/10.1016/S0024-3205(02)01858-1 [Google Scholar] [Crossref]
Grigorova, S., Kashamov, B., Sredkova, V., Surdjiska, S., & Zlatev, H. (2008). Effect of Tribulus terrestris extract on semen quality and serum total cholesterol content in white Plymouth rock-mini cock. Biotechnology in Animal Husbandry, 24(3-4),139-146. https://doi.org/10.2298/BAH0804139G [Google Scholar] [Crossref]
Haghmorad, D., Mahmoudi, M. B., Haghighi, P., Alidadiani, P., Shahvazian, E., Tavasolian, P., Hosseini, M., & Mahmoudi, M. (2019). Improvement of fertility parameters with Tribulus terrestris and Anacyclus pyrethrum treatment in male rats. International Brazilian Journal of Urology, 45(5),1043-1054. https://doi.org/10.1590/S1677-5538.IBJU.2018.0843 [Google Scholar] [Crossref]
Hassona, N., Zayed, M., Eltras, W. F., & Mohamed, R. A., (2020). Dietary supplementation of Tribulus terrestris extract improves growth and reproductive performances of the male Nile tilapia (Oreochromis niloticus). Aquaculture Research, 51(10), 4245-4254. https://doi.org/10.1111/are.14767 [Google Scholar] [Crossref]
Jayaprakas, V., & Bimal Lal, T. S. (1996). Factors affecting the motility and short-term storage of spermatozoa of the Indian major carps, Labeo rohita and Cirrhinus mrigala. Journal of Aaquaculture in the Tropics, 11, 67-78. [Google Scholar]
Kavitha, P., Ramesh, R., Bupesh, G., Stalin, A., & Subramanian, P. (2011). Hepatoprotective activity of Tribulus terrestris extract against acetaminophen–induced toxicity in a freshwater fish (Oreochromis mossambicus). In Vitro Cellular & Developmental Biology - Animal, 47, 698-706. https://doi.org/10.1007/s11626-011-9457-9 [Google Scholar] [Crossref]
Khaleghi, S., Bakhtiari, M., Asadmobini, A., & Esmaeili, F. (2017). Tribulus terrestris extract improves human sperm parameters in vitro. Journal of evidence-based complementary & alternative medicine, 22(3), 407-412. https://doi.org/10.1177/2156587216668110 [Google Scholar] [Crossref]
Kirk, R. E. (1968). Experimental design: Procedures for the behavioral sciences. Thomson Brooks/Cole Publishing Co. [Google Scholar]
Mann, H. B., & Whitney, D. R. (1947). On a test of whether one of two random variables is stochastically larger than the other. The Annals of Mathematical Statistics, 18(1), 50-60. [Google Scholar]
Meeni, I. M. (2016). Effect of alcoholic extract of Tribulus terrestris on fertility parameters in male rabbit. Medical Science, 5(4), 70-73. [Google Scholar]
Miller, A. L. (1996). Antioxidant flavonoids: structure, function and clinical usage. Alternative Medicine Review: A Journal of Clinical Therapeutic, 1(2), 103-111. [Google Scholar]
Moghaddam, M. H., Khalili, M., Maleki, M., & Ahmad Abadi, M. E. (2013). The effect of oral feeding of Tribulus terrestris L. on sex hormone and gonadotropin levels in addicted male rats. International Journal of Fertility & Sterility, 7(1), 57-62. [Google Scholar]
Platel, K., Rao, A., Saraswathi, G., & Srinivasan, K. (2002). Digestive stimulant action of three Indian spice mixes in experimental rats. Food/Nahrung, 46(6), 394-398. https://doi.org/10.1002/1521-3803(20021101)46:6<394::AID-FOOD394>3.0.CO;2-D [Google Scholar] [Crossref]
Pokrywka, A., Obminski, Z., Malczewska-Lenczowska, J., Fijałek, Z., Turek-Lepa, E., & Grucza, R. (2014). Insights into supplements with Tribulus terrestris used by athletes. Journal of Human Kinetics, 41, 99-105. https://doi.org/10.2478/hukin-2014-0037 [Google Scholar] [Crossref]
Rurangwa, E., Kime, D.E., Oliver, F., & Nash, J. P. (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234(1-4), 1-28. https://doi.org/10.1016/j.aquaculture.2003.12.006 [Google Scholar] [Crossref]
Sumalatha, K., Kumar, A. S., & Lakshmi, S. M. (2010). Review of natural aphrodisiac potentials to treat sexual dysfunction. International Journal of Pharmacy & Therapeutics, 1(1), 6-14. [Google Scholar]
Tekin, N. (1994). Spermanın muayenesi ve değerlendirilmesi: Evcil hayvanlarda reprodüksiyon, suni tohumlama, doğum ve infertilite. Dizgievi, 7, 69-79. [Google Scholar]
Yeganeh, S., Sotoudeh, A., & Movaffagh, A. N. (2017). Effects of Tribulus terrestris extract on growth and reproductive performance of male convict cichlid (Cichlasoma nigrofasciatum). Turkish Journal of Fisheries and Aquatic Sciences, 17(5), 1003–1007. https://doi.org/10.4194/1303-2712-v17_5_15 [Google Scholar] [Crossref]

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