Research article | Open Access
Acta Natura et Scientia 2025, Vol. 6(1) 46-54
Assessment of Grain Color Parameters With Functional and SSR Markers in Bread Wheat (Triticum aestivum L.)
pp. 46 - 54 | DOI: https://doi.org/10.61326/actanatsci.v6i1.352
Publish Date: June 23, 2025 | Single/Total View: 0/0 | Single/Total Download: 0/0
Abstract
This study aimed to examine the relationships between grain color parameters and functional markers of color-controlling genes in wheat. Grain color parameters (L*, a*, b*, C* and h°) of the segregated genotypes in the F2 generations of Byrd (red) × Tosunbey (white) and Kayra (red) × Cumhuriyet 75 (white) hybrids were measured. The F2 genotypes with parents were screened with wheat grain color SSR marker (Xgwm155) and functional color markers (Tamyb10-A1, Tamyb10-B1 and Tamyb10-D1). The color alleles possessed by the parents were determined by functional markers. While the parents with white color (Tosunbey and Cumhuriyet 75) were determined to be homozygous recessive in terms of all three-color alleles, Byrd variety with red grain color was found to have the R-B1b allele, and Kayra variety was found to have the red color allele in terms of all three loci. In terms of color parameters, the average color parameter values in the F1 generation and F2 population of both hybrid combinations gave results closer to the parent with red grain color. In the Byrd × Tosunbey F2 population, the interaction of two markers (Xgwm155 × Tamyb10-B1) in the L* color parameter and the Xgwm155 marker in the Hue angle (h°) parameter were found to be important and explained the observed variation by approximately 11%. In the Kayra × Cumhuriyet 75 F2 population, the Tamyb10-B1 marker in the L* parameter and Tamyb10-D1 in the b* and C* parameters were determined to be important and explained approximately 5% of the observed variation.
Keywords: Wheat, Grain color, Color parameters, Functional markers
APA 7th edition
Ozturk, S., Istipliler, D., & Tonk, F.A. (2025). Assessment of Grain Color Parameters With Functional and SSR Markers in Bread Wheat (Triticum aestivum L.). Acta Natura et Scientia, 6(1), 46-54. https://doi.org/10.61326/actanatsci.v6i1.352
Harvard
Ozturk, S., Istipliler, D. and Tonk, F. (2025). Assessment of Grain Color Parameters With Functional and SSR Markers in Bread Wheat (Triticum aestivum L.). Acta Natura et Scientia, 6(1), pp. 46-54.
Chicago 16th edition
Ozturk, Sevilay, Deniz Istipliler and Fatma Aykut Tonk (2025). "Assessment of Grain Color Parameters With Functional and SSR Markers in Bread Wheat (Triticum aestivum L.)". Acta Natura et Scientia 6 (1):46-54. https://doi.org/10.61326/actanatsci.v6i1.352
- Adams, K., Lukow, O. M., Suchy, J., DePauw, R., & Humphreys, G. (2013). Analysis of kernel colour, flour and whole wheat end-product quality of commercially grown Canada hard white spring wheat, snowbird. Journal of Agricultural Science, 5(1), 187-200. https://doi.org/10.5539/jas.v5n1p187 [Google Scholar] [Crossref]
- Aykut Tonk, F., İştipliler, D., Tosun, M., Turanli, F., İlbi, H., & Çakir, M. (2016). Genetic mapping and inheritance of Russian wheat aphid resistance gene in accession, IG 100695. Plant Breeding, 135(1), 21-25. https://doi.org/10.1111/pbr.12339 [Google Scholar] [Crossref]
- Bates, D., Mächler, M., Bolker, B., & Walker, S. (2015) Fitting linear mixed-effects models using lme4. Journal of Statistical Software, 67(1), 1-48. https://doi.org/10.18637/jss.v067.i01 [Google Scholar] [Crossref]
- Black, C. K., & Panozzo, J. F. (2004). Accurate technique for measuring color values of grain and grain products using a visible‐NIR instrument. Cereal Chemistry, 81(4), 469-474. https://doi.org/10.1094/CCHEM.2004.81.4.469 [Google Scholar] [Crossref]
- Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19(1), 11-15. [Google Scholar]
- Flintham, J. E. (2000). Different genetic components control coat-imposed and embryo-imposeddormancy in wheat. Seed Science Research, 10(1), 43-50. https://doi.org/10.1017/S0960258500000052 [Google Scholar] [Crossref]
- Guzman, C., Crossa, J., Mondal, S., Govindan, V., Huerta, J., Crespo-Herrera, L., Vrgas, M., Singh, R. P., & Ibba, M. I. (2022). Effects of glutenins (Glu-1 and Glu-3) allelic variation on dough properties and bread-making quality of CIMMYT bread wheat breeding lines. Field Crops Research, 284, 108585. https://doi.org/10.1016/j.fcr.2022.108585 [Google Scholar] [Crossref]
- Himi, E., Maekawa, M., Miura, H., & Noda, K. (2011). Development of PCR markers for Tamyb10 related to R-1, red grain color gene in wheat. Theoretical and Applied Genetics, 122(8), 1561-1576. https://doi.org/10.1007/s00122-011-1555-2 [Google Scholar] [Crossref]
- Himi, E., Mares, D. J., Yanagisawa, A., & Noda, K. (2002). Effect of grain colour gene (R) on grain dormancy and sensitivity of the embryo to abscisic acid (ABA) in wheat. Journal of Experimental Botany, 53(374), 1569-1574. https://doi.org/10.1093/jxb/erf005 [Google Scholar] [Crossref]
- Kaldy, M., Kereliuk, S., & Kozub, G. R. (1993). Influence of gluten components and flour lipids on soft white wheat quality. Cereal Chemistry, 70(1), 77-80. [Google Scholar]
- Lachman, J., Martinek, P., Kotíková, Z., Orsák, M., & Šulc, M. (2017). Genetics and chemistry of pigments in wheat grain. A review. Journal of Cereal Science, 74, 145-154. https://doi.org/10.1016/j.jcs.2017.02.007 [Google Scholar] [Crossref]
- Matus-Cadiz, M. A., Daskalchuk, T. E., Verma, B., Puttick, D., Chibbar, R. N., Gray, G. R., Perron, C. E., Tyler, R. T., & Hucl, P. (2008). Phenolic compounds contribute to dark bran pigmentation in hard white wheat. Journal of Agricultural and Food Chemistry, 56(5), 1644-1653. https://doi.org/10.1021/jf072970c [Google Scholar] [Crossref]
- McCallum, J. A., & Walker, J. R. L. (1990). Proanthocyanidins in wheat bran. Cereal Chemistry, 67(3), 282–285. [Google Scholar]
- McIntosh, R. A., Hart, G. E., Devos, K. M., Gale, M. D., & Rogers, W. J. (1998). Catalogue of gene symbols for wheat. Proceedings of the Ninth International Wheat Genetics Symposium, Vol.5, Saskatoon, Canada. pp. 1-229. [Google Scholar]
- Metzger, R. J., & Silbaugh, B. A. (1970). Location of genes for seed coat color in hexaploid wheat, Triticum aestivum L. Crop Science, 10(5), 495-496. https://doi.org/10.2135/cropsci1970.0011183X001000050012x [Google Scholar] [Crossref]
- Miyamoto, T., & Everson, E. H. (1958). Biochemical and physiological studies of wheat seed pigmentation. Agronomy Journal, 50, 733-734. https://doi.org/10.2134/agronj1958.00021962005000120005x [Google Scholar] [Crossref]
- Neuman, M. R., Sapirstein, H. D., Shwedyk, E., & Bushuk, W. (1989). Wheat grain colour analysis by digital image processing I. Methodology. Journal of Cereal Science, 10(3), 175-182. https://doi.org/10.1016/S0733-5210(89)80046-3 [Google Scholar] [Crossref]
- Peterson, C. J., Shelton, D. R., Martin, T. J., Sears, R. G., Williams, E., & Graybosch, R. A. (2001). Grain color stability and classification of hard white wheat in the US. Euphytica, 119, 101-107. https://doi.org/10.1023/A:1017515127628 [Google Scholar] [Crossref]
- R Core Team. (2021) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/ [Google Scholar]
- Rieseberg, L. H., Archer, M. A., & Wayne, R. K. (1999). Transgressive segregation, adaptation and speciation. Heredity, 83(4), 363-372. https://doi.org/10.1038/sj.hdy.6886170 [Google Scholar] [Crossref]
- Şahin, M., Akçura, M., Akçacık, A. G., & Doğan, S. (2006). Evaluation of color parameters with measured spectrophotometer in durum wheat breeding. Journal of Crop Research, 2(1), 17-21. [Google Scholar]
- Şanal, T., Olgun, M., Erdoğan, S., Pehlivan, A., Yazar, S., Başçiftçi, Z., Kutlu, İ., & Ayter, G. (2012). Quality analysis of Turkey in bread wheat by interpolation technique I. Red bread wheat. Biological Diversity and Conservation, 53, 69-75. [Google Scholar]
- Sherman, J. D., Souza, E., See, D., & Talbert, L. E. (2008). Microsatellite markers for kernel color genes in wheat. Crop Science, 48(4), 1419-1424. https://doi.org/10.2135/cropsci2007.10.0561 [Google Scholar] [Crossref]
- Slaughter, D. C., Norris, K. H., & Hruschka, W. R. (1992). Quality and classification of hard red wheat. Cereal Chemistry, 69(4), 428-432. [Google Scholar]
- Ünal, S. (2003). Buğday ve un kalitesinin belirlenmesinde uygulanan yöntemler. Nevşehir ekonomisinin sorunları ve çözüm önerileri: Un sanayi örneği. Nevşehir Ekonomi Sempozyumu, Nevşehir, Türkiye, pp. 15-33. [Google Scholar]
- Warner, R. L., Kudrna, D. A., Spaeth, S. C., & Jones, S. S. (2000). Dormancy in white-grain mutants of Chinese Spring wheat (Triticum aestivum L.). Seed Science Research, 10, 51-60. https://doi.org/10.1017/S0960258500000064 [Google Scholar] [Crossref]
